Device

Part:BBa_K1172903:Design

Designed by: Tore Bleckwehl   Group: iGEM13_Bielefeld-Germany   (2013-09-19)

Alanine racemase (alr) with double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 352
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 276
    Illegal BamHI site found at 978
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 394
    Illegal AgeI site found at 694
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 151


Design Notes

Cloning intermediate, which can be used also for further researches.

  • Classical [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Prefix_Insertion Prefix-Insertion] of the alanine racemase BBa_K1172901 into the plasmid backbone of the BioBrick BBa_B0015, containing the double terminator.
  • Result: RBS-alr-ter

Source

  • Genomic DNA Escherichia coli K-12
    • Isolation of the whole E. coli genome, see [http://2013.igem.org/Team:Bielefeld-Germany/Labjournal/ProtocolsPrograms#Whole_Genome_Isolation here]
  • BioBrick BBa_B0015 (double terminator)

References